General Information

  • ID:  hor001623
  • Uniprot ID:  Q9HCQ7
  • Protein name:  Neuropeptide RFRP-1
  • Gene name:  NPVF
  • Organism:  Homo sapiens (Human)
  • Family:  FARP (FMRFamide related peptide) family
  • Source:  Human
  • Expression:  Isoform 1 is specifically expressed in the retina. Neuropeptides RFRP-1 and NPVF are detected in the hypothalamus.
  • Disease:  Diseases associated with NPVF include Precocious Puberty, Central, 1.
  • Comments:  NA
  • Taxonomy:  Homo (genus), Homininae (subfamily), Hominidae (family), Hominoidea (superfamily), Catarrhini (parvorder), Simiiformes (infraorder), Haplorrhini (suborder), Primates (order), Euarchontoglires (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005102 signaling receptor binding; GO:0005515 protein binding
  • GO BP:  GO:0007218 neuropeptide signaling pathway; GO:0032277 negative regulation of gonadotropin secretion
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  MPHSFANLPLRF
  • Length:  12(81-92)
  • Propeptide:  MEIISSKLFILLTLATSSLLTSNIFCADELVMSNLHSKENYDKYSEPRGYPKGERSLNFEELKDWGPKNVIKMSTPAVNKMPHSFANLPLRFGRNVQEERSAGATANLPLRSGRNMEVSLVRRVPNLPQRFGRTTTAKSVCRMLSDLCQGSMHSPCANDLFYSMTCQHQEIQNPDQKQSRRLLFKKIDDAELKQEK
  • Signal peptide:  MEIISSKLFILLTLATSSLLTSNIFC
  • Modification:  T12 Phenylalanine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Act as a potent negative regulator of gonadotropin synthesis and secretion
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NPFFR1
  • Target Unid:   Q9GZQ6
  • IC50:  NA
  • EC50:  NA
  • ED50:  NA
  • Kd:  NA
  • Half life:  NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q9HCQ7-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor001623_AF2.pdbhor001623_ESM.pdb

Physical Information

Mass: 162608 Formula: C67H100N18O15S
Absent amino acids: CDEGIKQTVWY Common amino acids: FLP
pI: 10.55 Basic residues: 2
Polar residues: 2 Hydrophobic residues: 5
Hydrophobicity: 14.17 Boman Index: -966
Half-Life / Aliphatic Index: 30 hour Aliphatic Index: 73.33
Instability Index: 7788.33 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  20027225
  • Title:  Identification of human GnIH homologs, RFRP-1 and RFRP-3, and the cognate receptor, GPR147 in the human hypothalamic pituitary axis.